Victorian Cytology Service Lab
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Taking a Pap smear


+. Procedure for taking a Pap smear using the Cervex Sampler ||+. Pap Smear Flow Chart

Pre-Menopausal Women   Sampling Instruments
Cervex Sampler Broom: rotate 3-5 times
OR
Spatula: rotate once or twice, taking care to keep contact with the ecto-cervix
plus
Endocervical brush
: quarter rotation, then gently roll the brush onto the slide
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Peri & Post Menopause Women
Cervex Sampler Broom: rotate 3-5 times
O R
Spatula: rotate once or twice, taking care to
keep contact with the ecto-cervix
plus (with either method)
Endocervical brush: quarter rotation, then
gently roll the brush onto the slide.
Special Notes Techniques
1. Eversion: take care to sample the edge of the eversion. This is the squamocolumnar junction where the columnar epithelium of the endocervical canal meets the squamous epithelium of the vagina. It is the area where cell changes occur.

2. Pregnancy: do not use the Cytobrush
(endocervical brush) there is a remote
chance that it could cause membrane
damage.

3. After taking the sample, fix the slide immediately. Preferably within 4
seconds: definitely no longer than 30
seconds. The cells "expand" if they are
not fixed promptly and might appear
falsely abnormal.

4. If adding a ThinPrep sample, use the Cervex Sampler, or a plastic spatula. A wooden spatula should not be used. When you agitate the instrument(s) in the ThinPrep fluid you are trying to release as many cells as possible: cells seem to stick less to the plastic than the wooden spatula.

   

Labelling of Pap Test Slides            
 

To enable your patient's smear to be reported without delay please ensure the following patient identifiers are written in pencil on the slide.

  1. Patients First Name
  2. Patient Surname
  3. Patient Date of Birth


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